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Pulsed-Field Gel Electrophoresis (PFGE) PFGE was developed in 1984 and has since become the gold standard for bacterial subtyping.  PFGE is the primary subtyping method used by PulseNet, a network of public health laboratories that perform PFGE on foodborne disease organisms such as Salmonella, E. coli O157:H7, Shigella (11), Listeria
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